Vitamin b{11 {11 -enriched foods transformed from seed and endocermis

ABSTRACT

The gist of the present invention resides in a production method of nutrified foods particularly vitamin B12-enriched foods transformed from the seed germs, endodermis and medulla of vegetables characterized by blending crushed powders mainly constituting of vegetable seed germs and endodermis, to which vegetable medulla may be added if desired, further added with compounds of trivalent iron, calcium and magnesium and the like, blended and kneaded with an aqueous solution containing a small amount of an ammonium cobalt complex salt of an organic oxygenated acid and the like, or if desired, with said aqueous solution added with an iodine compound and fresh milk, sterilizing on heating by steaming, followed by cultivating thereon an edible mold and yeast to effect digestion and transformaton into edible and nutrified foods.

United States Patent [191 Tatara 1 1 Aug. 7, 1973 [76] lnventor: SeizoTatara, No. 18, Takdai-cho,

Funabashi, Japan [22] Filed: Nov. 13, 1970 [21] Appl. No.: 89,505

[52] US. Cl 99/11, 195/28 VB [51] Int. Cl A231 1/30 [58] Field of Search99/11, ,12, 83, 98, 99/99; 195/28 VB, 80

[56] References Cited UNITED STATES PATENTS 2,773,770 12/1956 Maki..-195/28 VB 3,012,217 2/1962 Zorn 99/2 3,085,049 4/1963 Rudym. 195/28 VB3,018,225 l/l962 Parlin 195/28 VB FOREIGN PATENTS OR APPLICATIONS649,226 9/1962 Canada 195/28 VB 637,769 3/1962 Canada 195/28 VB PrimaryExaminer- Norman Yudkoff Attorney-Steinberg & Blake [57] ABSTRACT Thegist of the present invention resides in a production method ofmuirified foods particularly vitamin B,

enriched foodstransformed from the seed germs, endodermis and medulla ofvegetables characterized by blending crushed powders mainly constitutingof vegetable seed germs and endodermis, to which vegetable medulla maybe added if desired, further added with compounds of trivalent iron,calcium and magnesium and the like, blended and kneaded with an aqueoussolution containing a small amount of an ammonium c0- balt complex saltof an organic oxygenated acid and the like, or if desired, with saidaqueous solution added with an iodine compound and fresh milk,sterilizing on heating by steaming, followed by cultivating thereon anedible mold and yeast to effect digestion and transformaton into edibleand nutrified foods.

3 Claims, No Drawings 1 VITAMIN Bm-ENRICHED FOODS TRANSFORMED FROM SEEDGERMS AND ENDOCERMIS SUMMARY OF THE'INVENTION The present inventionrelates to the production of nutrified foods, particularly vitamin B-enriched foods transformed from vegetable seed germs, endocermis, andmedulla, the non-edible wastes considered formerly into edible andhealthy, nutrified foods.

DESCRIPTION OF A PREFERRED EMBODlMENT',

According to the process of the present invention, the germ-containingendodermis of vegetable seeds such as rice, wheat, soya bean and thelike, after removal of the exodermis on thrashing and polishing thewastes of soya bean processing, rice-bran, wheat-bran, bean-curds huskand core medulla crashes of corn after thrashing, draff (bagasse) ofsugar cane and the like are ground and mixed, to the mixture is addedgreen leave powders, and an aqueous solution containing ammo niumcomplex salts of organic oxygenated acids suchas tartaric acid, malicacid, citric acid, etc., of essential minerals such as iron, calcium,magnesium and trace amounts of cobalt and a small amount of inorganicsalts, and an aqueous solution containing hot milk added with a smallamount of kalium triiodide, i.e., iodine containing milk, wherein theunsaturated fats and proteins have been added and combined with iodine,blended to dumps, steamed and sterilized by heating, mixed again aftercooling with seed microorganisms belonging to one or more species ofAspergillus and Saccharomyces, made into pellets and incubatedfor aspecified time intervals at an appropriate temperature to effect partialdigestion, thus obtained milky white dumps containing microbial cellsare dehydrated immediately by using a hot airor infrared ray-heatedvacuum drier to give solid matters, crushed and ground and sieved toremove remained coarse fibers, the resulted edible powders can beutilized alone or processed on addition of sugar into granules,chocolate forms or honey-candy drops as highly nutrient and healthyfoods containing various essential enzymes and vitamins as well asprotein.

The present process is now explained by the followmg embodiment:-

a. Fresh rice-bran containing germs 27 Kg b. Fresh wheat-bran containinggerms 25 Kg c. Crushed powders of corn cores 20 Kg 2 d. Dried powders ofconfry leaves 3 g a. Ferric ammonium citrate 250 g b. Calcium lactate150 g c. Magnesium malate 50 g d. Cobalt ammonium tartrate 5 g e. Ferricnitrate or ferric sulfate 60 g f. Fresh water 25 l a. Fresh milk 1 l b.Potassium triodide 1.5 g c. Ammonium malate 100 g d. Fresh water 50 l a.Aspergillus oryzae lOO g b. Aspergillus flavus 50 g c. Zygosaccharomycessoja 50 g According to the above formulation, the raw materials cited in(l) are freshly air-dried and mixed thoroughly. The minerals in (2) aredissolved in ca. 51, of fresh water in (f) successively in the order of(a), (b), (c), (d), and (e), made alkaline by adding a small amount ofammonia, finally made up to 501.

The starting materials described in (3) are prepared as follows: To llof fresh milk is added 0.5l of warm water, heated to maintain at aboutC, and then a solution of ca. 1.5 g of potassium triiodide dissolved isabout 100 cc of water is added therein gradually-with stirring whichresults in a milky white solution after iodination, after cooling onstanding, it-is mixed with a solution of 100 g of ammonium lamatedissolved in ll of water separately, made up to 25! with fresh water,and finally added with 50! of the solution prepared in (2), withstirring to give 75! of the processing solution. Thus obtained solutionis further adjusted at pH 7 by adding either 5 percent citric acidsolution or 10 percent aqueous ammonia, blended and kneaded on addinggradually into the raw materials of (i), made into clumps after thoroughimpregnation, steam-sterilized in an autoclave, and allowed to cool downto below 35C with continuous blending, and then mixed well with thegrown mycelia, dried and dehydrated as repidly as pos:

sible under 50C by using a hot air drier, or an infrared ray heatedvacuum drier, the dried matters are now.

finelydivided by a ball mill or an impulse grinder, sieved orpneumatically classified from unchanged coarse fiber to afford powdersof edible substances having much nutriments as shown in the followingtable and so can be utilized as excellent healthy, nutrient foods. l

Table. Results of Analysis (By Japan Food Analysis Center, Corp.)

Moisture 6.46 Crude protein l7.74 Crude fats and oils 2l.52 Sugars 34.333? Coarse fibers 9.04 Crude ashes l0.9l Calorific value 40l Cal/lOOVitamin E 24 2 mg% Vitamin B, l 51 pg% Vitamin B 0 25 mg% Calcium M959Sodium 1.18 Phosphorus 2 34 mg% Iron 3.84 Vitamin B. 4 32 mg% Vitamin B,0 64 mg% Nicotinic acid 25 3 p.g% Follic acid 33.9 mg% Pantothenic acid5.06 u.g% Biotin 44.3 Vitamin F 8.52 k Sucrose 2.23 Z1 Glucose 3.77Fructose 0 40 a-Amylase potency (D 40C 30) 4140 PU ,H, 422.5 CasFR/gretyr ,H. 780 0 ,H, 695.5

Protease potency Thus obtained product can be used directly or afterprocessing with sugar syrup, honey, or malt dextrose into granules,chocolate-like matter, or candy style healthy foods as it is compatiblewith those sugars.

Thus obtained powdery product'can also be used as a bathing agent aloneor in combination with other polysulfide bathing agents for the purposeof nourishing the skin, the nourishing and cosmetic effects areparticularly prominent in the combination with one of the conventionalbathing agents.

By the present invention, formerly abandoned wastes of vegetable seedgerm-containing endodermis such as rice-bran which contains much amountsand variety of vitamins, minerals and enzyme sources essential formaintenance of the growth is being mixed with porous powders of medullaof vegetables, useful for the growth of mycellia, thereon cultivatedwith molds (Aspergillus) and yeasts (Saccharomyces Zygosaccharomyces) toeffect partial digestion and transformation into edible matteraccompanied with formation of a variety of enzymes and vitamins. It isworthy to note that nevertheless, bio-synthesis of vitamin B has alreadybeen realized by liquid culture of a toxic species of Actinomyces(Strepto-myces olivaceus) in the presence of a trace amount of inorganicsalts of cobalt such as cobalt chloride; in contrast, according to thepresent invention, accumulation and enrichment of vitamin B can beeffected by cultivation of the said non-toxic, edible microorganism inthe presence of cobalt ammonium complex salts of organic oxygenatedcarboxylic acids, the said complex salts of cobalt being considered easytransformation into cyanocobalamin, namely vitamin B as reflected in thetable described above, though the mechanism in detail of the chemicalreaction is unknown, and so, non-edible waste matters can be changedinto effective healthy and nutrient foods thereby effectively.

I claim:

1. Method of converting normally non-edible vegetable waste materialinto edible food rich in vitamin B which comprises forming a crushedmixture of seed germs and endodermis, adding thereto an aqueous solutionof at least one complex salt of ammonium, an organic acid selecting fromthe group consisting of tartaric acid, malic acid, citric acid andlactic acid, and a metal selected from the group consisting of iron,calcium, magnesium and cobalt, and also adding thereto an aqueoussolution of hot iodinated milk, adding thereto seed microorganismsselected from the group consisting of the species of Aspergillus andSaccharomyces, incubating the thus formed mixture to obtain partialdigestion thereof, dehydrating the resulting mixture, thus obtaining asolid residue, crushing the thus remaining solid residue and removingcoarse fibers therefrom, thus obtaining an edible powder containingenzymes, vitamins and protein.

2. Method according to claim 1 wherein vegetable medulla is added tosaid mixture of seed germs and endodermis. I

3. Method according to claim 2 wherein said seed microorganisms areselected from the group consisting of Aspergillus oryzae, Aspergillusflavus, and Zygosaccharomyces sojae.

2. Method according to claim 1 wherein vegetable medulla is added tosaid mixture of seed germs and endodermis.
 3. Method according to claim2 wherein said seed microorganisms are selected from the groupconsisting of Aspergillus oryzae, Aspergillus flavus, andZygosaccharomyces sojae.